Evaluation of myopic cornea lenticules. A histochemical and clinical correlation.

In this work, we have analyzed the main clinical and corneal histological parameters that may be associated to the spherical equivalent (SE), age and gender of individuals with myopic refractive errors. For this purpose, 108 cornea stroma lenticules were obtained from patients subjected to ReLEx-SMILE myopia correction. Histological analyses were carried out and histochemistry and immunohistochemistry were used to quantify key histological components of the cornea stroma, including mature collagen fibers, reticular and elastic fibers, glycoproteins, proteoglycans, type-V collagen and several crystallins. Clinical and histological data were analyzed to determine their association with SE, age and gender. Results showed a significant correlation between the age range of the patients and the expression of crystallins CRY-α-A, CRY-λ1 and type-V collagen and between CRY-λ1 and corneal thickness, spherical diopters (D) and SE, although correlation between CRY-λ1 and SE was non-significant when age was controlled. Comparison of cases with low myopia and high/moderate myopia found statistical differences for D and lenticule thickness and diameter. The binary logistic regression analysis allowed us to construct a model using two clinical parameters (D and lenticule thickness). Parameters showing significant correlation with the age were the corneal radius, keratometry reading (K), OZ, CRY-α-A and type-V collagen, whereas SE, lenticule thickness, OZ, CRY-λ1 and type-V collagen showed statistically significant differences between the youngest and the oldest patients. A binary logistic regression analysis model was generated including 3 variables (D, cornea radius and OZ). No gender differences were found. The specific clinical and histological modifications found to be associated to the SE and age could be useful for a better understanding of the mechanisms involved in the genesis or progression of myopia and could establish the basement for future therapeutic options.

[Neuroretinitis. Clinical cases]. / Neurorretinitis. Casos clínicos.

OBJECTIVE: Evaluate the etiology and the most frequent funduscopic alterations of the neuroretinitis. METHODS: We present the case of a woman 24 years old with a bilateral decrease of visual sharpness (AV), painless and progressive, the funduscopic examination of which reveals a bilateral optical disc edema, with no hemorrhages or exudates, retina edema of the posterior pole and phlebitic areas. The systematic study was normal except for the hemogram (18,000/mm(3) leucocytes with 79% neutrophils) and the positive serology next to a Chlamydia. We also present the case of a 64 years old woman with a decrease of AV at the right eye of one week duration. At the back of the eye a macular star can be seen, and papilla edema. A systematic study gave normal results and positive serology at Bartonella henselae. RESULTS: The illness produced by a cat scratch is the most common cause of neuroretinitis. The customary findings are a loss of AV, discromatopsia, afferent papillary defects and abnormality on the visual field. Other frequent findings at the back of the eye are hemorrhagic nerve fibers, cotton-like exudates, papilla edema, macular star and glassy inflammation. The roll of antibiotic therapy is questionable. Oral ciprofloxacine seems to give good results.

Neurorretinitis. Casos clínicos / Neuroretinitis. Clinical cases

Objetivo: Describir la etiología y las alteraciones funduscópicas más frecuentes de las neurorretinitis.Métodos: Presentamos el caso de una mujer de 24años con disminución de la agudeza visual (AV)bilateral, indolora y progresiva cuyo examen funduscópicomostraba un edema de disco óptico bilateralsin hemorragias ni exudados, edema retinianoen polo posterior y zonas de flebitis. El estudio sistémicofue normal excepto el hemograma(18.000/mm3 leucocitos con 79% de neutrófilos) yla serología positiva frente a Chlamydia. Presentamostambién el caso de una mujer de 64 años deedad con disminución de la AV en el ojo derecho de1 semana evolución. En el fondo de ojo (FO) seaprecia una estrella macular y edema de papila.Resultó estudio sistémico normal y serología positivaa Bartonella henselae.Resultados: La enfermedad por arañazo de gato esla causa más común de neurorretinitis. Los hallazgoshabituales consisten en una pérdida de AV, discromatopsia,defecto pupilar aferente y anormalidadesen el campo visual. Otros hallazgos frecuentesen el FO son hemorragias en la capa de fibras nerviosas,exudados algodonosos, edema de papila,estrella macular e inflamación vítrea. El papel de laterapia antibiótica es cuestionable. Parece que elciprofloxacino oral tiene buenos resultados(AU)

Objective: Evaluate the etiology and the most frequentfunduscopic alterations of the neuroretinitis.Methods:We present the case of a woman 24 yearsold with a bilateral decrease of visual sharpness(AV), painless and progressive, the funduscopicexamination of which reveals a bilateral optical discedema, with no hemorrhages or exudates, retinaedema of the posterior pole and phlebitic areas. Thesystematic study was normal except for the hemogram(18,000/mm3 leucocytes with 79% neutrophils)and the positive serology next to a Chlamydia.We also present the case of a 64 years oldwoman with a decrease of AV at the right eye of oneweek duration. At the back of the eye a macular starcan be seen, and papilla edema. A systematic studygave normal results and positive serology at Bartonellahenselae.Results: The illness produced by a cat scratch is themost common cause of neuroretinitis. The customaryfindings are a loss of AV, discromatopsia,afferent papillary defects and abnormality on thevisual field. Other frequent findings at the back ofthe eye are hemorrhagic nerve fibers, cotton-likeexudates, papilla edema, macular star and glassyinflammation. The roll of antibiotic therapy is questionable.Oral ciprofloxacine seems to give goodresults(AU)

[Treatment of conjunctival intraepithelial neoplasia with topical mitomycin C 0.02%]. / Tratamiento de las neoplasias conjuntivales intraepiteliales con colirio de mitomicina C al 0,02%

PURPOSE: To evaluate the efficacy of topical mitomycin C (MMC) 0.02% in treating conjunctival intraepithelial neoplasia (CIN). METHODS: Three patients with CIN were treated with topical MMC 0.02%. Our follow-up period was twelve months (range 8-18 months). RESULTS: CIN was resolved in all three cases without modifying the normal corneal and conjunctival architecture. CONCLUSIONS: Topical MMC 0.02% four times daily during two weeks is a useful alternative tool for the surgical management of CIN.